The option was always available with v1.x, and day one with 2.0. But since yesterday it’s missing, so I’m forced to use FSR2 instead :-/
DLSS is available and working in other games. Nvidia GPU selected in-game, running full screen at native resolution.
I have multiple GPUs in my PC (laptop with TB eGPU) but my primary GPU is a NVIDIA GeForce RTX 4070 Ti SUPER. The other GPUs are Intel Iris Xe (iGPU) and NVIDIA RTX A2000 (dGPU).
I’m also using a 32:9 super ultrawide monitor (Samsung Odyssey OLED G9), connected to the eGPU.
Windows 11 24H2, with the latest NVIDIA drivers etc.
Blizzard, please fix this asap.
Update:
If I delete the LocalPrefs.txt settings file (in the Documents > Diablo IV folder) and start the game, DLSS is available to select. But when I close the game and start it again, the option is gone. So, this is definitely a new bug that was introduced yesterday.
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Same here. After I installed new nvidia drivers 506.03, dlss option was gone .
Rollback old drivers didn’t help. Now I see how radeon users suffer : )
But my 3090 is luckily strong enough to run in 4k without upscaling , just lower some settings from ultra to medium.At the moment I was scared that my gpu is dying 
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I have literally the same problem 
Same issue here and now the game lags and crashes…
Hello dear community
I have an rtx 3060 and the DLSS has disappeared for me too.
AND IT’S NOT NVIDIA OR WINDOWS’ FAULT
Unfortunately, I have to say that the new Vessel of Hatred is only causing problems and you really have to consider playing a different game. Especially since you can see once again who is being referred to here and who isn’t.
And another comment on the solution suggested by the first person:
It didn’t help at all, unfortunately.
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Yea ive been having the same problem. I use FSR 1 as i get higher FPS then using none for resolution scaling
For me, the DLSS option appeared on 6 Nov after the 2.0.4c game patch. Then it worked until yesterday, when the newest 2.0.5 patch was installed. Now it’s gone again and I’m forced to use XeSS or FRS2 
Your support only tells me there’s no workaround and to create a new bug report here…
Now I’ll stop playing until you can resolve this AGAIN…
Summary: The DLSS bug is back again in patch 2.0.5 Please fix it, again.
this happens pretty much every season or large patch, the option disappearing is them deliberately removing it because it’s causing issues. they disabled it with 2.0.2 and finally enabled it again in 2.0.4e.
for me it’s still there, so i don’t know why you don’t have it now, maybe they still having issues with some GPUs but not all or something?
to all in this thread: maybe look at the patch notes before posting? it’s literally the first sentence in there when they disable it